He has now won an unprecedented 10 consecutive Olympic or world championships gold medals across 10,000m and 5,000m.
Despite a couple of attempts during the race to push it, Farah was always in touch and it seemed inevitable he'd have too much speed over the last lap for the rest.
Rutherford wouldn't have been favourite to take gold but as defending champion, his absence certainly opens up the competition. It's all about Farah and how he races. "The guys gave it to me, it wasn't about Mo, it was about 'how do we beat Mo?'"
"I wasn't thinking I was going to lose, but I thought, "This is tough".
"I believe in clean sports and I just have to enjoy what I do and keep smiling". "It helped a lot having that experience".
Farah moved along in around 12th as Kamworor and compatriot Tanui injected more pace up front and the trio of Ethiopians loomed large on the scene.
All three Britons - CJ Ujah, James Dasaolu and Reece Prescod - will join him in the semi-finals, Prescod clocking a new personal best of 10.03 for third in his heat.
"I'm kind of sad I didn't get to go out here and get where I wanted to, but I guess I'll take something from it for next time". Anything can happen in long distance But you can't get carried away.
"I didn't want to go down. From that fall actually, I did hurt my leg". "I've got a few days now, to get ready".
His beloved Arsenal can only dream of a treble double but Sir Mo Farah heads to London on the cusp of more history in the 5,000m and 10,000m. "At championships you will see that gap, but whenever champs start people embrace them and just get behind athletics and who is there". He is halfway there in London.
Tomorrow morning, Olympic fourth-placer Thomas Barr kicks off in the heats of the 400m hurdles with the hope that he can match or improve on his performance in Rio. Viren is the only other man to do so twice, in 1972 and 1976, while Zatopek added the marathon for a unique treble in 1952. To have 10 global gold medals is incredible.
Chepng'etich has won two Diamond League meetings this season in Shanghai and 3:59.22 and 3:59.67, while Hassan cracked 3:56.22 and 3:57.10 in Rome and Paris respectively.
Four-time Olympic champion Farah will now aim to complete a triple-double at the World Championships when he competes in the 5,000 metres.
Kenya is yet to win men's World 10,000m title since Charles Kamathi's exploits at the 2001 Edmonton World Championships.
Farah hit the front with 650 metres to go but was challenged repeatedly in the closing stages.
Roared on by a capacity crowd as loud as anything experienced here during his Olympic triumphs five summers ago, the Briton nearly fell twice late in the race as an epic contest developed, before kicking down the home straight in trademark fashion to finally pull away.
Yet, five years to the very night when he won the 25-lap title at his home Olympics on "Super Saturday" in the same London Stadium, Farah had still not lost that invincible aura, clocking a searing 55.63 seconds for the last lap in spite of his stumbles.