Matsuyama carded a seven-under 64 to join Kisner at eight-under, two clear of Australian Jason Day, a former PGA champion, whose 66 saw him get to six-under and third position. Wang Jeunghun (73-75), Thongchai Jaidee (80-73) and Young-han Song (80-77) exited early.
Jason Day has surged into contention at the halfway point of the US PGA Championship in North Carolina.
Jason Day started looking like the player who was No. 1 for so much of previous year. Thorbjorn Olesen of Denmark and American Kevin Kisner led the way on four-under 67. The group only five shots behind featured Justin Thomas, Rickie Fowler and Paul Casey.
That's because the Aiken, S.C., native didn't have to worry about the inclement weather that suspended play on Friday afternoon at the PGA Championship. He followed with a bogey on the par-4 18th hole to make the turn at 1 over. The last three holes of Quail Hollow - 16th to 18th - are termed as the Green Mile and are among the toughest closing holes in golf. "I'm definitely picking it up", the world No. 3 said. "I'm just going to do the best I can and have as much fun as I can.' All of a sudden it falls in my lap".
Johnson started his round with a birdie on the par-5 10th, chipping from the rough to 4 feet, but he gave the stroke back on the next hole when he missed the green from 135 yards in the fairway and missed a 7-foot par putt.
"I wasn't hitting it like I wanted to", he told reporters via an interpreter.
But before that he bogeyed four times - on the first and then three in a row from seventh to ninth.
"I think what was making me exhausted was I wasn't hitting my driver like I wanted to".
"I'm sure everybody is happy it rained and softened up a little bit", Holmes said.
Matsuyama birdied his first hole after the break and made a two on the par-three 17th to take a share of the lead.
Day's nightmare round was epitomised by a unusual decision on the par-4 18th hole to go for the green with his approach after finding trouble from the tee.
Louis Oosthuizen shakes his right arm in discomfort after hitting a shot at the second hole while standing awkwardly against a tree.
The 28-year-old again displayed these characteristics of his recent form: streaky at times, but with the courage to pull himself back into the round.
Meanwhile, the favorite to win the competition before the start of Round One Rory McIlroy who struggled at the back nine by hitting a bogey in the 13th and a double bogey in the 14th.